We suggest that this locus could be related with the indirect response found in the GBS concentration. The results obtained suggest that modification in the SIN and GIB concentration by selection is related to the GSL-ALK locus. Divergent selections realized in leaves had a side effect in the GSL contents of flower buds and seeds due to the novo synthesis in these organs and/or translocation from leaves. Mass selection is an efficient way of modifying the concentration of individual GSLs. The divergent selection for GBS content was only successful and significant for decreasing the concentration, with a reduction of 39.04%. The content of SIN and GIB after three cycles of divergent selection increased 52.5% and 77.68%, and decreased 51.9% and 45.33%, respectively. The aims were to determine if the three divergent selections were successful in leaves, how each divergent selection affected the content of the same GSLs in flower buds and seeds and to determine which genes would be involved in the modification of the content of the three GSL studied. acephala) with high (HSIN, HIGIB, HGBS) and low (LSIN, LGIB, LGBS) content. We report the first results of a divergent selection for glucosinolate (GSL) content of the three major GSL in leaves: sinigrin (SIN), glucoiberin (GIB), and glucobrassicin (GBS) in order to develop six kale genotypes ( Brassica oleracea var. Modification of the content of secondary metabolites opens the possibility of obtaining vegetables enriched in these compounds related to plant defense and human health.
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